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Sally Gould's avatar

SO SORRY! HERE IS THE ABSTRACT, PLUS A PDF IS AVAILABLE The video is wonderful.

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https://f1000research.com/articles/9-943/v2 

A proposed molecular mechanism for pathogenesis of severe RNA-viral pulmonary infections [version 2; peer review: 4 approved]

Peter K. Rogan https://orcid.org/0000-0003-2070-52541,2, Eliseos J. Mucaki1, Ben C. Shirley2Author details

AbstractBackground: Certain riboviruses can cause severe pulmonary complications leading to death in some infected patients. We propose that DNA damage induced-apoptosis accelerates viral release, triggered by depletion of host RNA binding proteins (RBPs) from nuclear RNA bound to replicating viral sequences.

Methods: Information theory-based analysis of interactions between RBPs and individual sequences in the Severe Acute Respiratory Syndrome CoronaVirus 2 (SARS-CoV-2), Influenza A (H3N2), HIV-1, and Dengue genomes identifies strong RBP binding sites in these viral genomes. Replication and expression of viral sequences is expected to increasingly sequester RBPs - SRSF1 and RNPS1. Ordinarily, RBPs bound to nascent host transcripts prevents their annealing to complementary DNA. Their depletion induces destabilizing R-loops. Chromosomal breakage occurs when an excess of unresolved R-loops collide with incoming replication forks, overwhelming the DNA repair machinery. We estimated stoichiometry of inhibition of RBPs in host nuclear RNA by counting competing binding sites in replicating viral genomes and host RNA

.Results: Host RBP binding sites are frequent and conserved among different strains of RNA viral genomes. Similar binding motifs of SRSF1 and RNPS1 explain why DNA damage resulting from SRSF1 depletion is complemented by expression of RNPS1. Clustering of strong RBP binding sites coincides with the distribution of RNA-DNA hybridization sites across the genome. SARS-CoV-2 replication is estimated to require 32.5-41.8 hours to effectively compete for binding of an equal proportion of SRSF1 binding sites in host encoded nuclear RNAs. Significant changes in expression of transcripts encoding DNA repair and apoptotic proteins were found in an analysis of influenza A and Dengue-infected cells in some individuals.

Conclusions: R-loop-induced apoptosis indirectly resulting from viral replication could release significant quantities of membrane-associated virions into neighboring alveoli. These could infect adjacent pneumocytes and other tissues, rapidly compromising lung function, causing multiorgan system failure and other described symptoms.

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Sally Gould's avatar

THANK YOU  FOR SHARING YOUR ABSOLUTELY  FANTASTIC ARTICLES!!!

Here is an intriguing COMPUTER MODEL -GENERATED PREPRINT of a proposed mechanism by which COVID and possibly the vaccine could be causing the apoptosis of the cell and the spilling out of the infection into the body

.HTTPS://F1000RESEARCH.COM/ARTICLES/9-943/V2RESEARCH ARTICLEREVISED  A proposed molecular mechanism for pathogenesis of severe RNA-viral pulmonary infections

THRUST OF PROPOSAL:  "We propose an alternative mechanism in which infection of RNA virus triggers unrepaired sites of chromosomal breakage, causing apoptosis and consequentially, high-titer viral release.  This is precipitated by the binding of RNA binding proteins to viral genomes and transcripts instead of nuclear transcripts, to prevent destabilization of chromosome structure." -------------------------------------------------------------------------------------------

Plus, here is a link to a video discussion of this preprint, computer generated hypothesis:   https://www.youtube.com/watch?v=6jNo0on8vhg&t=486s

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